| Primary Identifier | MGI:6781956 | Allele Type | Targeted |
| Gene | F2r | Transmission | Germline |
| Strain of Origin | B6.Cg-Thy1<a> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A mutation was engineered in exon 2 to change arginine codon 41 (CGC) to a glutamine codon (CAA)(p.R41Q) and an FRT site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent flp-mediated recombination. The mutation prevents cleavage at that residue by thrombin or activated protein C (APC). |
