| Primary Identifier | MGI:6887963 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Inheritance Mode | Dominant | Transmission | Germline |
| Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using ES cells containing the RMCE-ready Gt(ROSA)26Sortm1Rkuhn allele, the original cassette was exchanged with a vector containing the following elements: an attB site, the CAG promoter, a loxP site flanked puromycin resistance gene cassette, a YFP gene in-frame with a human DROSHA cDNA with a p.E1147K mutation and C-terminal FLAG tag sequences, and a second attB site. The mutated Drosha will only be expressed after cre-mediated deletion of the puro cassette. The mutation is associated with Wilms Tumor and critically affects the metal-binding Drosha needs for its cleaving activity. |
