| Primary Identifier | MGI:6885604 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Ttr |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology using two gRNAs, one for exon 1 and one for exon 4, and a single donor vector carrying the whole gene in which mouse exons were replaced with human exons but with mouse introns, replaced the endogenous gene with this exon-humanized TTR gene. The a6221 clone showed germline transmission and no gross rearrangement or deletion and sequencing confirmed that all exons have human sequencing. |
