| First Author | Ouyang L | Year | 2022 |
| Journal | iScience | Volume | 25 |
| Issue | 1 | Pages | 103604 |
| PubMed ID | 35005549 | Mgi Jnum | J:321431 |
| Mgi Id | MGI:6854197 | Doi | 10.1016/j.isci.2021.103604 |
| Citation | Ouyang L, et al. (2022) miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3alpha in bone. iScience 25(1):103604 |
| abstractText | Coordination between osteogenesis and angiogenesis is required for bone homeostasis. Here, we show that miR-29cb2 is a bone-specific miRNA and plays critical roles on angiogenesis-osteogenesis coupling during bone remodeling. Mice with deletion of miR-29cb2 exhibit osteopenic phenotypes and osteoblast impairment, accompanied by pronounced decreases in specific H vessels. The decrease in bone miR-29cb2 was associated with pathological ovariectomy stimuli. Mechanistically, hypoxia-inducible factor (HIF)-3alpha, as a target for miR-29cb2, inhibits HIF-1alpha activity by competitively bonding with HIF-1beta. Notably, miR-29cb2 in peripheral blood (PB) nearly is undetectable in sham and significantly increases in ovariectomy mice. Further evaluation from osteoporosis patients demonstrates similar signatures. ROC analysis shows miR-29cb2 in PB has higher sensitivity and specificity for diagnosing osteoporosis when compared with four clinical biomarkers. Collectively, these findings reveal that miR-29cb2 is essential for bone remodeling by inhibiting HIF-3alpha and elevated bone-specific miR-29cb2 in PB, which may be a promising biomarker for bone loss. |
