| Primary Identifier | MGI:6887630 | Allele Type | Targeted |
| Attribute String | Endonuclease, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | C57BL/6NTac x 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | C57BL/6NTac x 129S6/SvEvTac ESCs |
| molecularNote | A BAC containing the Gt(ROSA)26Sor gene (BAC_ESr2-445b1_sfi_1) was modified by replacing part of intron 1 with a loxP site flanked neomycin resistance gene cassette, the Cas9 gene, sequence for P2A self-cleaving peptide sequence and the GFP reporter gene. The BAC replaced the endogenous locus through homologous recombination. The neo cassette was removed through subsequent cre-mediated recombination, leaving an allele expressing Cas9 and GFP driven by the Gt(ROSA)26Sor promoter. |
