| Primary Identifier | MGI:7279294 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Nckap1l |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector PG00089_Y_3_F01 from KOMP was was electroporated into ES cells. The construct is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 12-16 at position 103382899. The critical exons 12-16 are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene leaving exons 12-16 floxed. |
