| Primary Identifier | MGI:7260126 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Mettl5 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S6/SvEvTac)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A Mettl5 conditional knockout line was made in which exon 2 was floxed, such that the presence of Cre would induce exon 2 deletion by creating a frame-shift mutation. An FRT-flanked neomycin cassette included in the construct was excised. The construct was electroporated with gRNA/Cas9 protein into ES cells that are F1 hybrid of C57bl/6 x 129S6. |
