| First Author | Kinoshita N | Year | 2022 |
| Journal | iScience | Volume | 25 |
| Issue | 2 | Pages | 103846 |
| PubMed ID | 35198899 | Mgi Jnum | J:321248 |
| Mgi Id | MGI:6885208 | Doi | 10.1016/j.isci.2022.103846 |
| Citation | Kinoshita N, et al. (2022) Force-dependent remodeling of cytoplasmic ZO-1 condensates contributes to cell-cell adhesion through enhancing tight junctions. iScience 25(2):103846 |
| abstractText | The physiological importance of biomolecular condensates is widely recognized, but how it is controlled in time and space during development is largely unknown. Here, we show that a tight junction protein ZO-1 forms cytoplasmic condensates in the trophectoderm (TE) of the mouse embryo before E4.0. These disappear via dissolution, and ZO-1 accumulates at the cell junction as the blastocyst cavity grows and internal pressure on TE cells increases. In contrast, this dissolution was less evident in TE cells attached to the inner cell mass because they receive weaker tensile forces. Furthermore, analyses using MDCK cells demonstrated that the ZO-1 condensates are generated and maintained by liquid-liquid phase separation. Our study also highlights that the dynamics of these condensates depends on the physical environment via an interaction between ZO-1 and F-actin. We propose that the force-dependent regulation of ZO-1 condensation contributes to the establishment of robust cell-cell adhesion during early development. |
