| Primary Identifier | MGI:7275270 | Allele Type | Endonuclease-mediated |
| Gene | Ins2 | Strain of Origin | NOD |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Guide RNA and a mutant ssDNA oligonucleotide template are designed to create a CGT to a GAG mutation, resulting in an arginine to glutamic acid substitution at position 46 (R46E) in the insulin B chain (B22) of the insulin II (Ins2) gene [R(B22)E]. A silent mutation was also added, creating an Alu I restriction site. |
