| Primary Identifier | MGI:7278809 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Optn |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Glutamic acid codon 50 (GAG) in exon 2 was changed to lysine (AAG) (c.148G>A p.E50K) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This mutation, in the EMO (NF kappa B essential modulator) domain of the encoded peptide, replicates the same human mutation associated with normal-tension glaucoma (NTG). |
