| First Author | Zeng XN | Year | 2007 |
| Journal | Mol Cell Neurosci | Volume | 34 |
| Issue | 1 | Pages | 34-9 |
| PubMed ID | 17074507 | Mgi Jnum | J:123216 |
| Mgi Id | MGI:3717515 | Doi | 10.1016/j.mcn.2006.09.008 |
| Citation | Zeng XN, et al. (2007) Aquaporin-4 deficiency down-regulates glutamate uptake and GLT-1 expression in astrocytes. Mol Cell Neurosci 34(1):34-9 |
| abstractText | The role of aquaporin-4 in water transport has been extensively investigated, while little information exists regarding its contribution to astrocytic functions such as the action to glutamatergic transmission. Since aquaporin-4 has been detected widely co-localized with glutamate transporter 1 (GLT-1) and glutamate transporters also present water transport properties, we investigated the regulative role of aquporin-4 on glutamate transporter using primary cultured astrocytes from aquaporin-4 knockout (AQP4(-/-)) mice. It was demonstrated that lack of aquaporin-4 down-regulated astrocytic expression of GLT-1 but not of glutamate/aspartate transporter (GLAST). The result from [(3)H]D,L-glutamate uptake analysis showed a lower uptake capability in AQP4(-/-) astrocytes. Furthermore, MTT and LDH assays indicated less cellular toxicity induced by excessive glutamate in AQP4(-/-) genotype. These findings provide direct evidences for the first time that aquaporin-4 plays an important role in the function of glutamate transporters. And the present study will improve our understanding of aquaporin-4-glutamanergic biology. |
