| Primary Identifier | MGI:7261348 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Slc12a5 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site, a neomycin resistance gene cassette, and a second loxP site, were inserted 5' to exon 5, and a third loxP site was inserted 3' to exon 5. Exon 5 encodes the end of the second transmembrane domain (TM2) and a portion of the intracellular loop between TM2âTM3. Deletion of this exon results in a preterm stop codon and complete KCC2 knockout. The neomycin cassette was removed via Cre-mediated recombination in the germline of mice, leaving loxP sites flanking exon 5. |
