| Primary Identifier | MGI:7263364 | Allele Type | Endonuclease-mediated |
| Attribute String | Not Specified | Gene | Idh1 |
| Strain of Origin | 129S6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using CRISPR-Cas9 technology in Idh1em1Nbar zygote, a floxed STOP cassette (Lox-STOP-Lox) into the mouse genomic Idh1 locus upstream of a modified exon 3 engineered to contain a nucleotide substitution in codon 132 to cause the conversion of LSL-Idh1R132H allele to the LSL-Idh1R132C. Four sequence- and (R)-2HGâvalidated founder lines were selected for further experimental study. This is a representative line used for the study. |
