| Primary Identifier | MGI:7264674 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cant1 |
| Transmission | Germline | Strain of Origin | C57BL/6J x 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A G to A transition at position 905 (c.G905A) resulting in an arginine to histidine substitution at amino acid 302 (p.R302H) was introduced in exon 4. In addition, an FRT-flanked neomycin selection cassette and loxP site were inserted upstream of exon 3 and a second loxP site was inserted downstream of mutated exon 4. The selection cassette was removed via flp-mediated recombination and cre-mediated recombination deleted exons 3 and 4. Real-time PCR confirmed absence of mRNA in skin. |
