Primary Identifier | MGI:7281348 | Allele Type | Transgenic |
Attribute String | Epitope tag, Inserted expressed sequence | Gene | Tg(Thy1-Gabrg2/EYFP)1Amcr |
Strain of Origin | Not Specified | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Site-directed mutagenesis and PCR were used to add His and FLAG epitopes (ASGHHHHHHGMDYKDDDDKGLG) to the c-teminus of a cDNA for rat GABAA receptor γ2 subunit (GABAARγ2), short form. Enhanced YFP was inserted between amino acids 4 and 5 of the mature γ2 peptide coding sequence. A mouse Thy1 promoter drives expression of the construct. |