| Primary Identifier | MGI:7310435 | Allele Type | Endonuclease-mediated |
| Gene | Antxr1 | Strain of Origin | C57BL/6Ncr x B6D2F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Two guide RNAs (5'-TCATCATCGCGTTGACGGAT-3', PAM=GGG; 5'- GACGGATGGGGAGCTGCACG-3', PAM=AGG) and a single-stranded DNA containing the E150V mutation is used to create a GAG to GTA missense mutation. A silent mutation was also added, creating a ScaI restriction site (AGTACT). This mutation hinders the binding of ANTRX1 to collagen. |
