| Primary Identifier | MGI:7311667 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Rarres1 |
| Transmission | Germline | Strain of Origin | 129S1/SvImJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeted KO-first, conditional ready, lacZ-tagged mutant vector PRPGS00072_A_A02 from the KOMP project 39930 was obtained from UC Davis KOMP-CSD and was used to insert an FRT site followed by, splice acceptor site, IRES, lacZ sequence with polyadenylation sequence, and a loxP site, followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site into intron 2 of the gene. A third loxP site is inserted into intron 3. The critical exon is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene, leaving exon 3 floxed. Exon 3 was deleted via cre-mediated recombination in the germline. |
