| Primary Identifier | MGI:7327126 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Gtf2i |
| Transmission | Germline | Strain of Origin | C57BL/6NCr |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to insert a cassette containing floxed exons 15-34 and a mutated endogenous exon 15 downstream of the cassette. Specifically, the vector included, from 5' to 3', a loxP site, splice acceptor, cDNA encoding exons 15-34, a STOP cassette, a PGK-neo cassette, and a second loxP site downstream of endogenous exon 14. Downstream of the cassette, endogenous exon 15 was modified to harbor a Chr. 7: 74146970 T to A substitution, resulting in a leucine to histidine mutation (L424H), identified as the most frequent mutation in human thymic epithelial tumors (TETs). |
