Primary Identifier | MGI:7482790 | Allele Type | Endonuclease-mediated |
Attribute String | Not Specified | Gene | Mgp |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Serine codons 22 (TCT), 25 (AGC) and 28 (TCC) were targeted for mutation to alanine codons (GCT, GCC, GCT) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. This allele contains all three mutations (p.S22A, p.S25A, p.S28A (p.S3A, p.S6A, p.S9A in mature protein)). The mutations block phosphorylation of the residues in the encoded peptide. |