Primary Identifier | MGI:7482647 | Allele Type | Endonuclease-mediated |
Attribute String | Inserted expressed sequence, Reporter | Gene | Cdkn1c |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using CRISPR/Cas9 system, the T2A-DTR-T2A-Venus cassette was inserted in-frame into the endogenous Cdkn1c (p57) coding sequence immediately 5â to the only TGA codon at this locus in ES cells. Single colony ES cell clones that had undergone homologous recombination were then expanded and injected into C57BL/6 mouse blastocysts to generate chimeric mice. |