| Primary Identifier | MGI:7341397 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Trp53 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An arginine to tryptophan (C-to-T change at position 733 and C-to-G change at position 735) substitution was introduced at codon 245 (p.R245W) in exon 7. In addition, a loxP site, Trp53 exons 7-11 with a native polyadenylation signal sequence, and FRT site flanked PGK neomycin resistance gene cassette and second loxP site were inserted in intron 6. The PGK neomycin cassette was removed via Flp-mediated recombination. The floxed exons 7-11 were removed via cre-mediated recombination in the germline resulting in the expression of the R245W mutation. |
