Primary Identifier | MGI:7382781 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready | Gene | H2-K1 |
Transmission | Germline | Strain of Origin | (B6.129P2-Trp53<tm1Brn>/J x B6(129X1)-Dst<em13Dcr>/DcrJ)F1 |
Is Recombinase | false | Is Wild Type | false |
description | ES cells = KP*1 created by crossing B6.129P2-Trp53 |
molecularNote | CRISPR/cas9 genome editing technology is used to insert a cre-inducible affinity tag, StrepTagII, into intron 1 of the gene. The vector included two pairs of inward-facing heterospecific Lox sites (loxP and lox2272) flanking an inverted StrepTagII. |