| Primary Identifier | MGI:7339241 | Allele Type | Targeted |
| Gene | Clcn3 | Transmission | Germline |
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | An A to C change resulting in a glutamate to alanine substitution at amino acid 224 (p.E224A) was introduced in exon 5. Additionally, a neomycin resistance cassette flanked by FRT sites was introduced between exon 5 and 6. The neomycin cassette was removed via flp-mediated recombination. This is an uncoupling point mutation in the gating glutamate, a pore residue critically involved in 2Cl-/H+-exchange. |
