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Allele : Isl1<em1Vave> ISL1 transcription factor, LIM/homeodomain; endonuclease-mediated mutation 1, Vasanth Vedantham
Primary Identifier  MGI:7367280 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag Gene  Isl1
Strain of Origin  FVB/NJ Is Recombinase  false
Is Wild Type  false
molecularNote  Sequence coding for three FLAG tags was inserted in-frame immediately upstream of the stop codon using sgRNAs (targeting CATGGTAGCCAGTCCTATTG and TCCTCATGCCTCAATAGGAC) and an ssODN template (ATCGATGTCCTCGCAGCTCCCAGATACACCCAACAGCATGGTAGCCAGTCCTATTGAAGCAGGTGGTGGTGGTTCTGACTACAAAGACCATGACGGTGATTATAAAGATCATGATATCGATTACAAGGATGACGATGACAAGTGAGGAACATTCATT) using CRISPR/Cas9 technology.
  • mutations:
  • Insertion
  • synonyms:
  • Isl1<3xFLAG>,
  • Isl1<3xFLAG>
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

2 Publication categories

Trail: Allele




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