| First Author | Sakamoto M | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 2 | Pages | 101421 |
| PubMed ID | 35693213 | Mgi Jnum | J:347231 |
| Mgi Id | MGI:7616622 | Doi | 10.1016/j.xpro.2022.101421 |
| Citation | Sakamoto M, et al. (2022) In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice. STAR Protoc 3(2):101421 |
| abstractText | We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022). |
