| Primary Identifier | MGI:7398205 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mir22 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | BAC clone CH29-40010 containing the mouse Mir22 gene was manipulated through Red/ET recombination technology. A loxP was inserted in the 5â flanking region of exon 1 and a second loxP site was introduced in the 3â flanking region of exon 1 with a PGK-FRT-Neo-FRT-loxP cassette. Targeted ES cells were re-electroporated with a flp-recombinase expressing plasmid to remove the neomycin cassette leaving exon 1 floxed. Exon 1 was deleted via cre-mediated recombination in the germline. RT-qPCR confirmed undetectable levels of mature miR-22-3p in the liver, epididymal white adipose tissue and gastrocnemius tissue. |
