| Primary Identifier | MGI:7398299 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Clcn2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 2 and 3 were flanked by loxP sites and a neomycin-resistance cassette flanked by FRT sites was inserted between exons 1 and 2. To prevent missplicing, a putative splice acceptor site downstream of the neomycin cassette was mutated (gt to ca) and an additional EcoRV-site was introduced adjacent to the second loxP site to aid in Southern blot detection. The neomycin cassette was removed via flp-mediated recombination. |
