| Primary Identifier | MGI:7424899 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Rpl10 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 5 to 7 were flanked by loxP sites and an FRT-flanked selection cassette was inserted upstream of the second loxP site (downstream of exon 7). Downstream of the floxed region, exon 5 containing an arginine to serine substitution at amino acid 98 (p.R98S) was inserted. Prior to cre recombination, the wild-type gene is expressed. Cre-mediated recombination can be used to remove the floxed region which results in expression of the mutant protein. The selection cassette was removed via flp-mediated recombination. |
