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Allele : Mov10<em1(IMPC)Tcp> Mov10 RISC complex RNA helicase; endonuclease-mediated mutation 1, The Centre for Phenogenomics

Primary Identifier  MGI:7425836 Allele Type  Endonuclease-mediated
Attribute String  Null/knockout Gene  Mov10
Inheritance Mode  Not Specified Strain of Origin  C57BL/6NCrl
Is Recombinase  false Is Wild Type  false
Project Collection  IMPC
molecularNote  This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of TGTGGTGCTAGGATAACCTA targeting the 5' side and TATAGGCACATTCAGCTCGC targeting the 3' side of a critical region (ENSMUSE00000173482, ENSMUSE00000173486, ENSMUSE00000173485, ENSMUSE00000173480 and ENSMUSE00000173487) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 3,107-bp deletion (Chr3:104,707,954 to 104,711,060, GRCm39) with an insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCGGT).
  • mutations:
  • Intragenic deletion
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1 Feature

Trail: Allele

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

Trail: Allele

0 Driven By

3 Publication categories

Trail: Allele