| Primary Identifier | MGI:7411386 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Tbc1d12 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTGACTTATGCAAATGTTT and TTTCACTATGTACAAATTTG, which resulted in a 413 bp deletion beginning at Chromosome 19 position 38,865,432 bp and ending after 38,865,844 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000319961 (exon 2) and 289 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 247 and early truncation 31 amino acids later. |
