Primary Identifier | MGI:7433257 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Ank3 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector is created using the flp excision system (FLEx). The vector contains from 5'; to 3': lox2272, loxP sites, a FLEx cassette containing the last exon of Ank3 (ENSMUSE00001264329 within ENSMUST00000182992.8), an FRT-flanked neo cassette, a pGHpA sequence, and an EGFP reporter in reverse orientation followed by lox2272 and loxP sites inserted downstream of the endogenous last exon. Flp-mediated recombination removed the neo cassette. |