| Primary Identifier | MGI:7427724 | Allele Type | Targeted |
| Attribute String | Conditional ready, Recombinase | Gene | Kcnip2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct incorporated the sequence of an optimized roxed-Cre recombinase sequence preceded by the sequence coding for a short ââself-cleavingââ peptide P2A, and followed by an FRT-flanked neomycin resistance gene (Neo) into the third coding exon of the Kcnip2 gene. The insert carries an intersectional Dre-dependent Cre recombinase sequence achieved through the inclusion of a STOP cassette flanked by a pair of rox sites into the cre sequence. In presence of dre recombinase, cre recombinase is expressed driven by Kcnip2. The neo-selection sequence was removed by crossing chimeric mice with Flpe deleter mice. |
