Primary Identifier | MGI:7413116 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(RP23-43G17)delta7.1Jeng |
Strain of Origin | (C57BL/6J x SJL)F2 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The transgene insert contains the following elements: an FRT site flanked neomycin resistance gene cassette, Gata3 promoter 1b (-1314 to +1 relative to ATG start site; contains downstream promoter, alternative 1st exon 1b, intron 1b and 5' UTR part of exon 2), the EGFP reporter gene cDNA, and BAC RP23-43G17 (which contains Gata3 T cell and thymic NK cell enhancer (7.1 kb KpnI-SalI T cell element) Rr338) where Rr338 was replaced with an FRT site flanked neomycin resistance gene cassette. The neo cassettes were removed through subsequent flp-mediated recombination. EGFP expression is not observed in thymocytes anymore. |