| Primary Identifier | MGI:7439767 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Pot1a |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An arginine to cysteine substitution at amino acid 117 was knocked-in using the FLEx switch system. A cassette containing the following elements replaced the wild-type exon 7: a loxP site, a loxP511 site, exon 7 with a mutation changing arginine codon 117 (CGC) to cysteine (TGC) (p.R117C) in reverse orientation, an FRT site flanked neomycin selection cassette, a loxP site in reverse orientation, wild-type exon 7 in forward orientation, and a loxP511 site in reverse orientation. Upon expression of Cre recombinase, the wild-type exon 7 will be removed and the mutated exon, harboring the R117C missense mutation, will be flipped to the forward orientation. |
