| Primary Identifier | MGI:7439139 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified regulatory region | Gene | Rr381 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The Zbtb7b silencer was targeted with a dsODN template, coding for the first 250 bp of the silencer sequence in reverse orientation, using TALEN technology (ZFN target sequences (capitalized) ACCGCTACCCTAACCcataaCTGGAAGGGGTTTAG). The resulting allele has the 5' (distal) end of the silencer flipped, while the 3' (proximal) end, containing a highly conserved noncoding element (HCNE), remains in the normal orientation. |
