| Primary Identifier | MGI:7442684 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Irak4 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | InGeniousâ TruView Conditional Knockout System was used to generate this conditional allele. Exons 2 to 12 were flanked by loxP sites and split green fluorescent protein (GFP) cassettes. The FRT-flanked neomycin selection cassette was removed at the embryonic stem cell stage via inGeniousâ proprietary FLP embryonic stem cells. Upon cre-mediated recombination and deletion of the targeted region, the two GFP cassettes come together and express under the CAG promoter on the antisense strand. |
