| Primary Identifier | MGI:7464270 | Allele Type | Endonuclease-mediated |
| Attribute String | Not Specified | Gene | Got2 |
| Strain of Origin | either: C57BL/6J or (C57BL/6J x C3H/HeJ)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 8 was targeted with an sgRNA (targeting TCCTGACTTCTCCAGACTTG) and an ssODN template (CCGTCCCCTGTATTCCAACCCACCTCTCAATGGGGCCCGGATCGCAGCAACCATTCTGACGTCTCCGGATTTAGGTAAGCAATGGTAACGATTACTAGCTGTTCCGTGCTACAGCTCCATGAATGGAAAAG) using CRISPR/Cas9 technology to change arginine codon 337 (CGG) to glycine (GGT) (p.R337G, c.1009_1011CGG>GGT). The equivalent human mutation (p.Arg337Gly c.1009C>G) is found in some Malate-Aspartate Shuttle (MAS)-Related Encephalopathy patients. |
