| Primary Identifier | MGI:7463984 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Atl1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An AA to GC change resulting in a lysine to alanine substitution at amino acid 80 (p.K80A) was introduced in exon 3. In addition, a loxP site was inserted upstream of exon 3 and an FRT-flanked neomycin resistance cassette and second loxP site were inserted downstream of exon 3. The neomycin resistance cassette was removed via flp-mediated recombination. The p.K80A missense changes abolishes its GPTase activity. qPCR indicates that mRNA levels are similar to wild-type levels, however mutant protein levels in the brain and spinal cord are moderately lower. |
