| Primary Identifier | MGI:7450840 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Guf1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATACAGAATCATTATTACAG and TGTCCAGTGGACATGGTATG, which resulted in a 377 bp deletion beginning at Chromosome 5 position 69,561,071 bp and ending after 69,561,447 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001240940 (exon 7) and 312 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 206 and early truncation 4 amino acids later. |
