| Primary Identifier | MGI:7468731 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Idh1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site, engrailed 2 (En2) splice acceptor sequence, cDNA for Idh1 exons 3-9, SV40 poly(A) signal sequences, a second loxP site, an FRT site, a neomycin resistance gene cassette and a second FRT site were inserted into intron 2 and a p.R132H (arginine 132 to histidine) mutation was introduced in exon 3. The neo cassette was removed through subsequent Flp-mediated recombination and the cDNA was removed through Cre-mediated recombination. |
