| Primary Identifier | MGI:7481982 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Esr1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A wild-type Esr1 exon 10 cDNA, followed by a 4x polyadenylation signal, an FRT-flanked PGK-neomycin selection marker, a second loxP site, and a mutated exon 10 containing a tyrosine to serine substitution at amino acid 541 (p.Y542S) replaced exon 10. The PGK-neomycin cassette was removed via flp-mediated recombinase. This corresponds to Y537S, one of the most commonly occurring human ESR1 mutations. Cre-mediated recombination is necessary to remove the wild-type exon 10 and allow expression of the modified exon 10. |
