| Primary Identifier | MGI:7482299 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Myh4 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| description | A single targeting event using a gRNA targeting exon 3 introduced simultaneous frameshift mutations into Myh1 and Myh4 generating a strain with both a Myh1 nucleotide deletion (Myh1em1Ktsu) and a Myh4 nucleotide insertion (Myh4em1Ktsu) (J:335578). |
| molecularNote | CRISPR/Cas9 technology using gRNA 5â-CACCACAAACACCGATGACTTGG-3â targeting exon 3 (the first coding exon) of both Myh1 and Myh4 genes generated a single nucleotide insertion in Myh4. This targeting event also generated a single nucleotide deletion in Myh1 (Myh1em1Ktsu) in this line. |
