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Allele : Gt(ROSA)26Sor<em3(CAG-Insm1,-tdTomato)Zyliu> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Zhiyong Liu
Primary Identifier  MGI:7505567 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Epitope tag, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  (C57BL/6 x DBA/2J)F1 Is Recombinase  false
Is Wild Type  false
molecularNote  Plasmids encoding a guide RNA (ACTCCAGTCTTTCTAGAAGA) were designed to insert a cassette encoding a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG) followed by a floxed STOP cassette and a 3xHA (hemaglutinin) C-terminal tagged Insm1 gene into the Gt(ROSA)26Sor locus. A viral 2A oligopeptide (T2A) self cleaving peptide, that mediates ribosomal skipping, fused to a red fluorescent protein sequence (tdTomato) was inserted downstream.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26<Insm1>,
  • Rosa26-CAG-Loxp-stop-Loxp-Insm1*3×HA-P2A-tdTomato,
  • Rosa26<Insm1>,
  • Rosa26-CAG-Loxp-stop-Loxp-Insm1*3×HA-P2A-tdTomato
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0 Mutation Involves

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