| Primary Identifier | MGI:7539540 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Igh |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129P2/OlaHsd)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting vector consisted of a 4.1-kb long 50 homology region (containing Cmu1 to CmuS), a frt-flanked 2.1-kb DNA fragment containing the mouse phosphoglycerate kinase (Pgk1) promoter linked to the neomycin (Neo) resistance gene and a SV40 polyadenylation signal, a 1.6-kb DNA fragment containing the CmuM1/2 sequence linked in frame via the P2A peptide to an iCre gene followed by a 1.8-kb long 30 homology region was used to insert P2A-iCrE gene after the CmuM1/2 exons of the Igh gene . The frt-flanked Pgk1-Neo expression cassette was deleted by Flpe recombinase. |
