| Primary Identifier | MGI:7509779 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Ctcf |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was designed to insert a loxP-flanked PMC1-neomycin cassette upstream of exon 3 and a loxP-flanked PGK-puromycin cassette followed by a splice acceptor sequence and a lacZ reporter downstream of exon 12. ES cells were isolated de novo from these Ctcf conditional mice, and infected with lentivirus expressing cre recombinase to remove floxed-neo and floxed-puro. ES cells with a single loxP upstream of an exon 3 and another single loxP after the exon 12 essentially flanking the exons 3-12 with lacZ reporter downstream, were selected. |
