| Primary Identifier | MGI:7509491 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Smg7 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting cassette was inserted upstream of the critical exon 2. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. The fisrt loxP site is followed by a neomycin resistance gene under the control of the beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2. The critical exon 2 is thus flanked by loxP sites. The lacZ and neomycin resistance gene were removed via flp-mediated recombination leaving exon 2 floxed. |
