| Primary Identifier | MGI:7718776 | Allele Type | Targeted |
| Attribute String | Conditional ready, Recombinase, Reporter | Gene | Vim |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting vector was designed with (from 5'to 3') an inward facing lox2272 site upstream of exon 2 of the vimentin(Vim) gene, followed by exons 3 and 4, and an inverted cassette flanked with inward facing loxP sites. The inverted floxed cassette contains a polyadenylation sequence, a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), a Flp-recombinase (FlpO), T2A sequence, enhanced green fluorescent protein (EGFP), a human HIST1H2BB gene [histone 1 H2bb], a splice acceptor site and a second lox2272 site in opposite orientation to the first, followed by an FRT-flanked neomycin cassette and a second inward facing loxP site all downstream of exon 4. |
