Primary Identifier | MGI:7495884 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Tssk3 |
Strain of Origin | B6D2F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 genome editing technology was used to generate a 1551 bp deletion in the gene, corresponding to the entire region encoding the kinase domain, and almost the entire protein coding region except the first 6 amino acids. Western blot analysis confirmed the absence of encoded protein in homozygous mutant testes. |