Primary Identifier | MGI:7513906 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Mafb |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using recombination-mediated genetic engineering, a loxP-EM7-Neo-loxP ligated to Mafb 5' segment and an FRT-Neo-FRT-loxP ligated to Mafb 3' segments were introduced into mouse embryonic stem cells. Successfully recombined clones were injected into blastocysts to make loxP-flanked Mafb-Neo mice and crossed to the FLP-expressing line to remove the Pgk-Neo cassette. |