| Primary Identifier | MGI:7514372 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Syngap1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo cassette was inserted in intron 20 and point mutations disrupting the PDZ-binding motif were inserted in exon 21 via homologous recombination. Cre mediated recombination removed the neo cassette. Western blot analysis detected no difference in expression levels compared to the wild-type protein. Co-immunoprecipitation assays confirmed the loss of association between the mutant protein and Dlg4. This modification disrupts the function of the alpha 1 isoform while leaving expression of the other isoforms intact. |
